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. Author manuscript; available in PMC: 2014 Jul 1.
Published in final edited form as: Nat Methods. 2013 Dec 8;11(1):73–78. doi: 10.1038/nmeth.2762
Buffer A (store at 4°C):
Final Concentration Stock concentration Amount used from stock
15 mM Tris-Cl, pH 8.0 1 M Tris-Cl, ph 8.0 15 ml
15 mM NaCl 5 M NaCl 3 ml
60 mM KCl 1 M KCl 60 ml
1 mM EDTA, pH 8.0 0.5 M EDTA, pH 8.0 2 ml
0.5 mM EGTA, pH 8.0 500 mM EGTA, pH 8.0 1 ml

add sterile ddH2O to a final volume of 1 liter.

make fresh Buffer A+: to 10ml buffer A add the following