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. Author manuscript; available in PMC: 2014 Jul 1.
Published in final edited form as: Nat Methods. 2013 Dec 8;11(1):73–78. doi: 10.1038/nmeth.2762
10X DnaseI Digestion Buffer (Roche)
For 10ml (pH7.9)
Final concentration Stock concentration Amount used from stock
60 mM MgCl2 1 M MgCl2 600 ul
100 mM NaCl 5 M NaCl 200ul
10 mM CaCl2 1 M CaCl2 100 ul
400mM Tris-HCl 1 M Tris-HCl 4ml