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| Stop Buffer (per Liter) | ||
| Final concentration | Stock concentration | Amount used from stock |
| 50 mM Tris-Cl, pH 8.0 | 1 M Tris-Cl, pH 8.0 | 50 ml |
| 100 mM NaCl | 5 M NaCl | 20 ml |
| 0.10 % SDS | 20% SDS | 5 ml |
| 100 mM EDTA, pH 8.0 | 0.5 M EDTA, pH 8.0 | 200 ml |
Combine stock solutions and add sterile ddH20 to a final volume of 1 Liter. Dispense into 50-mL aliquots and store at 4 C. (SDS will precipitate at 4°C but will go back into solution upon heating to 55 °C)
