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. Author manuscript; available in PMC: 2014 Jul 1.
Published in final edited form as: Nat Methods. 2013 Dec 8;11(1):73–78. doi: 10.1038/nmeth.2762
Make fresh, for 10ml stop buffer, add
Spermine 0.1M 0.3mM 30ul
Spernidine 0.1M 0.1mM 100ul