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. 2014 May 12;205(3):357–375. doi: 10.1083/jcb.201308146

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© 2014 Wang et al.

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Figure 3. — Rng8 and Rng9 are critical for Myo51 localization. (A) Colocalization of Myo51 and Rng8 in the contractile ring (arrow), cables (arrowheads), and puncta (asterisks). The imperfect colocalization on the cables is likely caused by the highly dynamic nature and movement of the structure. (B) Myo51 localization is essentially abolished without either Rng8 or Rng9. Arrows indicate weak signals left at the contractile ring in some cells. (C) Localization of Rng8 and Rng9 in myo51Δ. Bars, 5 µm. (D) Rng8 and Rng9 coIP with Myo51. Broken lines indicate that intervening lanes have been spliced out. (E) FRAP analysis of Myo51 in the contractile ring. Curve fit is shown in red, SD in gray. (F) Numbers of Myo51 molecules in the whole cells and in the contractile ring. The fluorescence intensity of Myo51 was compared with that of Rng8. Error bars indicate 1 SD.