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. 2014 May 12;205(3):377–393. doi: 10.1083/jcb.201309096

Figure 1.

Figure 1.

CHC association with α-actinin2 in adult skeletal muscle. (A) Confocal sections of mouse skeletal muscle were processed for double-immunofluorescent labeling of DNM2 (red) and α-actinin (green) or CHC (red) and α-actinin (green). Bars, 10 µm. (B) The fluorescence intensity as a function of distance on the fiber was plotted for eight successive striations, and was reported on the graph for each labeling. The green curve corresponds to the green immunolabeling, and the red curve corresponds to the red labeling. (C and D) CHC localization in mouse dissociated FDB fibers. (C) Confocal sections of fibers processed for immunofluorescent labeling with the monoclonal antibody against CHC (X22, green). Bars: (main panel) 10 µm; (inset) 2 µm. (D) XZ and YZ projections of confocal optical sections illustrated in C. Bars, 10 µm. (E) Immunoblot of proteins associated with CHC, α-actinin (isoform 2), vinculin, or control immunoprecipitates from mouse muscle lysates and 1–5% lysate input. Bands for coimmunoprecipitated CHC, α-actinin (isoform 2), vinculin, talin, or CLC are indicated at the right.