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. 2014 May 12;205(3):409–428. doi: 10.1083/jcb.201308031

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© 2014 Alcaraz et al.

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Figure 8. — TSP-1 is not required for FBG-mediated activation of latent TGF-β. (A) Immunoblots showing phospho-Smad2 (P-Smad2) levels in NMuMG cells cultured for 3 h with (+) or without (−) soluble purified TSP-1 (11 nM) in the presence of neutralizing anti–TSP-1 antibody (α-TSP1) or normal serum IgG (10 µg/ml). (B) Levels of phosphorylated Smad2 in NMuMG cells cultured for 3 h on noncoated (N-C) or 222 pmol/cm² FBG-coated dishes or treated with 5 ng/ml of mature TGF-β1 in the presence of neutralizing anti–TSP-1 antibody as described in A. (C) Levels of phospho-Smad2 in NMuMG cells cultured for 3 h with (+) or without (−) 11 nM of soluble purified TSP-1 in the presence of a competing (LSKL) or control (SLLK) peptide (10 µM). (D) Phospho-Smad2 levels in NMuMG cells cultured for 3 h on noncoated (−) or FBG-coated (+) dishes, in the absence (−) or presence of competing or control peptide as described in C.