Table 1.

Oligonucleotide primers used for quantitative real-time RT-PCR

Gene	Primer sequence (strand)	Product size	Temperature	PCR cycles	Reference or accession no.
		bp	°C
E-cadherin (Cdh1)	5′-CTGCGCTGGATAGTGTGTGT-3′ (+)	187	58	40	NM_009864
	5′-TGGCATGCACCTAAGAATCA-3′ (−)
Keratin-19 (Krt19)	5′-GATCAGCGGTTTTGAAGCCC-3′ (+)	195	58	40	NM_008471
	5′-GTCTCGCTGGTAGCTCAGAT-3′ (−)
Mucin-1 (Muc1)	5′-AGACCCCAGCTCCAACTACT-3′ (+)	178	58	40	NM_013605
	5′-TGACTTCACGTCAGAGGCAC-3′ (−)
N-cadherin (Cdh2)	5′-GAGAGGCCTATCCATGCTGA-3′ (+)	204	58	40	NM_007664
	5′-CGCTACTGGAGGAGTTGAGG-3′ (−)
Vimentin (Vim)	5′-GTGCGCCAGCAGTATGAAAG-3′ (+)	110	58	40	NM_011701
	5′-GCATCGTTGTTCCGGTTGG-3′ (−)
Fibronectin (Fn1)	5′-CCCAGACTTATGGTGGCAATTC-3′ (+)	200	58	40	NM_010233
	5′-AATTTCCGCCTCGAGTCTGA-3′ (−)
Hmga2	5′-AGCAAAAACAAGAGCCCCTCTA-3′ (+)	100	58	40	Thuault et al., 2006
	5′-ACGACTTGTTGTGGCCATTTC-3′ (−)
Snail1 (Snai1)	5′-CCACTGCAACCGTGCTTTT-3′ (+)	66	58	40	Thuault et al., 2008
	5′-CACATCCGAGTGGGTTTGG-3′ (−)
Zeb1	5′-ACAAGACACCGCCGTCATTT-3′ (+)	120	58	40	Thuault et al., 2008
	5′-GCAGGTGAGCAACTGGGAAA-3′ (−)
Gapdh	5′-TGTGTCCGTCGTGGATCTGA-3′ (+)	76	58	40	Valcourt et al., 2007
	5′-CCTGCTTCACCACCTTCTTGA-3′ (−)

Accession numbers were obtained from GenBank.