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. Author manuscript; available in PMC: 2014 May 13.
Published in final edited form as: Science. 2013 Oct 11;342(6155):253–257. doi: 10.1126/science.1242088

Fig. 3. The GWAS-marked BCL11A enhancer is sufficient for adult-stage erythroid expression.

Fig. 3

(A) A 12.4-kb fragment of BCL11A intron-2 (+52.0–64.4 kb from TSS) was cloned to a lacZ reporter construct. Transient transgenic mouse embryo from 12.5 dpc X-gal stained. Arrowhead indicates liver.

(B) Cell suspensions isolated from peripheral blood (PB) and fetal liver (FL) of stable transgenic embryos at 12.5 dpc X-gal stained.

(C) Sorted erythroblasts and B-lymphocytes from young adult stable transgenic mice subject to X-gal staining or RNA isolation followed by RT-qPCR. Gene expression normalized to GAPDH and expressed relative to T-lymphocytes. Error bars indicate s.d.