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. Author manuscript; available in PMC: 2015 Mar 17.
Published in final edited form as: Cancer Cell. 2014 Mar 17;25(3):282–303. doi: 10.1016/j.ccr.2014.02.025

Table 3.

Anti-ERBB combinations

Combination Mechanism(s) of action Relevant clinical trials
Trastuzumab + lapatinib (or neratinib, afatinib) ADCC, partial disruption of HER2-HER3 dimers, inhibition of HER2 and EGFR tyrosine kinases (Baselga et al., 2012a; Blackwell et al., 2010)
Trastuzumab + pertuzumab (only approved combination) More complete inhibition of ligand-induced and ligand-independent HER2-containing heterodimers, ADCC, downregulation of HER2 from cell surface (Baselga et al., 2012b; Gianni et al., 2012; Schneeweiss et al., 2013)
T-DM1 + pertuzumab Same as above plus inhibition of polymerization of microtubules with DM1 MARIANNE (NCT01120184)
Trastuzumab + everolimus ADCC, disruption of ligand-independent HER2-HER3 dimers, inhibition of TORC1 BOLERO-3 (NCT01007942)
Trastuzumab + pertuzumab + PI3K inhibitor Inhibition of ligand-induced and ligand-independent HER2-containing heterodimers, ADCC, ATP-competitive inhibition of catalytic activity of p110 Pending
Trastuzumab + HER3 neutralizing antibody ADCC, partial disruption of HER2-HER3 dimers, inhibition of heregulin binding, downregulation of HER3 and/or HER3 dimerization Pending
Trastuzumab + HER3 antibody + PI3K inhibitor Same as above plus direct inhibition of p110 Pending
T-DM1 + PI3K inhibitor ADCC, partial disruption of HER2-HER3 dimers, inhibition of polymerization of microtubules, direct inhibition of p110 Pending
Afatinib + cetuximab Combined targeting of EGFR T790M to compensate for complete inhibition of target by either approach alone. Afatinib may also target resistance due to HER2 activation Pending
EGFR + PI3K inhibitor Block resistance due to re-activation of PI3K signaling Pending
Erlotinib + MET inhibitor Block MET dependent resistance to EGFR inhibitors MetLung Trial and others
EGFR inhibitor + IGF-IR antibody Block IGF-IR dependent resistance to EGFR inhibitors Pending
Erlotinib + Hydroxychloroquine Effort to block the survival of “drug tolerant” cells following treatment with EGFR TKIs (Sharma et al, Cell, 2010) (Goldberg et al., 2012)
Irreversible EGFR inhibitor + MET inhibitor Overcome both T790M and MET mediated resistance Pending