TABLE 2.

Steady-state kinetic parameters of CaADH^a

Substrate	kcat (s−1)	Km (mM)	kcat/Km (M−1 s−1)
Acetaldehyde	93 ± 6	5.5 ± 1.4	1.7 × 104
Acetoin	145 ± 4	71 ± 5	2.0 × 103
2R,3R-Butanediol	2.1 ± 0.2	65 ± 12	3.3 × 101
meso-2,3-Butanediol			<1
2S,3S-Butanediol	ND	ND	ND
Acetone	51.4 ± 0.8	0.60 ± 0.02	8.6 × 104
Butanone	44 ± 2	1.2 ± 0.1	3.8 × 104

^aND, not detected.