TABLE 3.
Capability of various strains to reduce exogenous acetone to isopropanol
| Organism | Growth medium | Concn (mM) upon acetone addition |
Concn (mM) 120 h after acetone addition |
OD600 120 h after acetone addition | ||
|---|---|---|---|---|---|---|
| Acetone | Isopropanol | Acetone | Isopropanol | |||
| Acetogens | ||||||
| A. woodii DSM 1030 | PETC, pH 8.2 | 186 ± 15 | 0 | 186 ± 17 | 0 | 0.33 ± 0.05 |
| C. aceticum DSM 1496 | PETC, pH 7.4 | 173 ± 5 | 0 | 174 ± 10 | 0 | 0.31 ± 0.04 |
| C. autoethanogenum DSM 10061 | PETC, pH 5.9 | 159 ± 7 | 0 | 19 ± 17 | 138 ± 15 | 0.49 ± 0.03 |
| C. carboxidivorans DSM 15243 | PETC, pH 5.9 | 180 ± 12 | 0 | 178 ± 12 | 0 | 0.54 ± 0.07 |
| C. ljungdahlii DSM 13528 | PETC, pH 5.9 | 176 ± 10 | 0 | 64 ± 19 | 112 ± 17 | 0.47 ± 0.02 |
| C. ragsdalei ATCC BAA-622 | PETC, pH 5.9 | 194 ± 17 | 0 | 171 ± 28 | 22 ± 21 | 0.41 ± 0.05 |
| Isopropanol producer | ||||||
| C. beijerinckii NRRL-B593 | RCM | 171 ± 3 | 0 | 132 ± 29 | 43 ± 17 | 1.32 ± 0.06 |
| Acetone producers | ||||||
| C. beijerinckii NCIMB 8052 | RCM | 181 ± 10 | 0 | 182 ± 7 | 0 | 1.44 ± 0.08 |
| C. acetobutylicum ATCC 824 | RCM | 186 ± 10 | 0 | 188 ± 10 | 0 | 1.61 ± 0.12 |
| Controls | ||||||
| E. coli DH5α | LB | 201 ± 15 | 0 | 202 ± 17 | 0 | 1.82 ± 0.11 |
| Blank medium | PETC | 181 ± 7 | 0 | 182 ± 12 | 0 | |
| RCM | 179 ± 12 | 0 | 183 ± 12 | 0 | ||
| LB | 192 ± 16 | 0 | 194 ± 9 | 0 | ||