TABLE 3.

Characterization of growth of M. extorquens DM4 and mutants with MMA when provided with plasmid copies of GMAS homologs in trans

Interruptedb CDS(s) and plasmidc	Avg generation time (h) ± SD with MMAa provided as:
	C + N source	C source	N source
None
pCM80	18.9 ± 0.9	18.0 ± 0.4	4.4 ± 0.2
pME8280	16.2 ± 0.6	15.3 ± 0.3	3.3 ± 0.2
pME8285	17.5 ± 0.9	18.8 ± 1.6	3.5 ± 0.2
METDI2327
pCM80	No growth	No growth	42.6 ± 1.2
pME8280	16.9 ± 0.7	15.9 ± 0.3	4.7 ± 0.3
pME8285	20.2 ± 0.8	16.5 ± 1.3	4.0 ± 0.3
METDI2327, METDI4690
pCM80	No growth	No growth	41.7 ± 1.2
pME8285	21.5 ± 0.5	19.2 ± 1.5	4.0 ± 0.1

^aMMA was added as the C or N source at 20 or 1.5 mM, respectively. In controls, succinate at 5 mM and (NH₄)₂SO₄ at 1.5 mM were provided as C and N sources, respectively. Similar growth of all strains on succinate (5 mM) and (NH₄)₂SO₄ (1.5 mM) was observed (generation time, 3.4 ± 0.3 h).

^bInterruption of METDI4690 alone conferred no phenotype with MMA as the C or N source (Table 2, mutant DM4Δmetdi4690).

^cpCM80, empty expression vector; pME8280, pCM80 for gmaS expression of METDI2327; pCM8285, pCM80 for expression of METDI4690.