TABLE 1.

Primers used for the detection of F+ RNA phages

Primer	Sequence (5′–3′)	Tmb	Product length (bp)
FRNA-I-Ra	CTTGTTCAGCGAACTTCTTRTA	53	142
FRAN-I-Fa	CAAACCAGCATCCGTAGCC	57
FRNA-II-R	GAAAACAAACCGTTGCCG	53	183
FRAN-II-F	GGTTCAAGTTGCGGGATG	55
FRNA-III-Ra	TTACGATTGCGAGAAGGCTG	57	115
FRAN-III-Fa	CCGCGTGGGGTAAATCC	57
FRNA-IV-R	TTCCAGCCRGGCTCGAT	57	183
FRAN-IV-F	CGTGGAAGCATGCCTGT	57

^aPrimer selected from previous studies (9, 20).

^bT_m, melting temperature.