TABLE 3.
Naturally occurring F+ RNA phages in environmental samples
| Sample type | Sample no. | Avg (SD) titer of F+ RNA phages (log10 PFU ml−1) |
||
|---|---|---|---|---|
| Plaque assay | RT-PCR | Two-step ET RT-PCR | ||
| Raw sewage | 1 | 3.23 (0.05) | 4.23 (0.15) | 3.68 (0.12) |
| 2 | 3.38 (0.09) | 4.49 (0.18) | 3.94 (0.23) | |
| 3 | 3.42 (0.05) | 4.80 (0.16) | 3.79 (0.19) | |
| 4 | 3.50 (0.06) | 4.61 (0.23) | 3.81 (0.19) | |
| 5 | 3.19 (0.08) | 5.04 (0.17) | 3.61 (0.21) | |
| 6 | 3.16 (0.11) | 5.09 (0.13) | 3.46 (0.13) | |
| 7 | 3.56 (0.05) | 3.91 (0.20) | 3.39 (0.17) | |
| 8 | 3.14 (0.13) | 4.36 (0.16) | 3.41 (0.16) | |
| Primary treated wastewater | 9 | 3.01 (0.13) | 4.26 (0.06) | 3.74 (0.14) |
| 10 | 3.04 (0.14) | 4.79 (0.17) | 3.31 (0.22) | |
| 11 | 2.92 (0.07) | 4.78 (0.23) | 3.89 (0.12) | |
| 12 | 2.93 (0.07) | 4.57 (0.22) | 3.72 (0.14) | |
| 13 | 2.82 (0.05) | 4.99 (0.25) | 3.44 (0.12) | |
| 14 | 2.77 (0.09) | 5.04 (0.22) | 3.56 (0.12) | |
| 15 | 3.20 (0.09) | 3.99 (0.21) | 3.45 (0.12) | |
| 16 | 2.88 (0.05) | 4.33 (0.28) | 3.43 (0.16) | |