FIG 3.

Influence of Lo18 on the fluidity of liposomes derived from B. subtilis or L. lactis or of synthetic lipids. Liposome fluidity is expressed in anisotropy R values as a function of the temperature in the absence (filled symbols) or presence (open symbols) of 10 μM Lo18. Liposomes were composed of DPPG (A), DPPE (B), DOPG (C), or DOPE (D) or lipids extracted from B. subtilis (E) or L. lactis (F) cytoplasmic membrane. The mass ratio between protein and liposomes was 1:2. The experiments were done in triplicate; vertical bars indicate standard deviations. Asterisks show statistically significant differences (P < 0.05).