Skip to main content
. 2014 May;80(10):2973–2980. doi: 10.1128/AEM.04178-13

FIG 4.

FIG 4

Influence of liposomes on the oligomerization of Lo18. Elution profiles of Lo18 loaded onto a Superdex 200 column. Prior to analysis, samples were incubated at 42°C for 30 min with or without liposomes. (A) Lo18 oligomerization in the presence of control liposomes. Profile 1 (dotted line), Lo18 alone; profile 2, Lo18/liposome ratio (m/m) of 1:2; profile 3, Lo18/liposome ratio of 1:10. Results of the column calibration are displayed. The inset shows the results of cross-linked Lo18 in the presence of control liposomes (Lo18/liposome ratios of 1:2 and 1:10). (B) Elution profile of Lo18 alone (dotted line) and Lo18 incubated with ethanol liposomes (Lo18/liposome ratios [m/m] of 1:2 and 1:10; black lines, similar profiles). In each panel, arrows indicate changes to the elution profiles upon the addition of liposomes. UOD, units of optical density.