FIG 5.

Chaperone activity of Lo18 in the presence of liposomes. Light scattering at 360 nm, which is a measure of the thermal aggregation of 300 nM citrate synthase (CS) at 45°C, was recorded as a function of time in the absence (filled circles) or presence of 9.6 μM Lo18 (open circles). (A) The dose-dependent inhibition of chaperone activity caused by increasing amounts of control liposomes (light gray squares, 12.5 μg; medium gray squares, 92.5 μg; dark gray squares, 925 μg; black squares, 9,250 μg). (B) The dose-dependent inhibition of chaperone activity caused by increasing amounts of ethanol liposomes (light gray diamonds, 925 μg; dark gray diamonds, 9,250 μg). (C) The inhibition of chaperone activity caused by liposomes composed of phospholipids extracted from membranes of B. subtilis (gray crosses, 9,250 μg) or L. lactis (gray triangles, 9,250 μg). The mean of three experiments is represented; vertical bars indicate standard deviations.