TABLE 3.
Implementation of plasmid addictiona
| Strain | Final ethanol titer (g/liter) | Final OD600 | Yield (g ethanol/g glycerol) | Specific glycerol consumption rate (g/liter/h/OD) | Specific ethanol production rate (g/liter/h/OD) |
|---|---|---|---|---|---|
| GB5/pGB1002 | 40.8 | 8.5 | 0.44 | 0.15 | 0.067 |
| GB6/pGB1002 | 5.4 | 3.7 | 0.28 | 0.07 | 0.020 |
| GB6/pGB1003 | 41.9 | 16.9 | 0.37 | 0.09 | 0.034 |
a
Experiments for GB5/pGB1002 and GB6/pGB1002 were conducted in Sartorius Biostat A Plus 7-liter reactors. GB5/pGB1002 and GB6/pGB1002 were grown in MM29 minimal medium at pH 6.3 and a kLa of 16/h for 72 h. The experiment for GB6/pGB1003 was conducted in Sartorius Biostat Q Plus 0.75-liter reactors. GB6/pGB1003 was grown in MM29 minimal medium at pH 6.3 and a kLa of 60/h for 72 h.