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Mass spectrometry analysis of purified GFP6×His. The top and bottom bands of purified GFP6×His (Fig. 3B) were isolated and subjected to in-gel digestion with chymotrypsin. The digests were analyzed by LC-MS/MS to identify specific peptides corresponding to GFP6×His. (A and B) Peptide coverages of the top and bottom bands of purified GFP6×His. The sequence identified by LC/MS-MS is in boldface and is underlined within the PhoDSP-GFP6×His sequence.
