TABLE 1.
Primers, probes, and plasmid target constructs
| Oligonucleotide | Sequence (5′ → 3′) | Reference |
|---|---|---|
| HF183 | ATCATGAGTTCACATGTCCG | 2 |
| BFDRev | CGTAGGAGTTTGGACCGTGT | 3 |
| BFDFam | FAM-CTGAGAGGAAGGTCCCCCACATTGGA-TAMRAa | |
| BacR287 | CTTCCTCTCAGAACCCCTATCC | This study |
| BacP234MGB | FAM-CTAATGGAACGCATCCC-MGB | This study |
| BacP234IAC | VIC-AACACGCCGTTGCTACA-MGB | This study |
| Standard | ATCATGAGTTCACATGTCCGCATGATTAAAGGTATTTTCCGGTAGACGATGGGGATGCGTTCCATTAGCTCGAGATAGTAGGCGGGGTAACGGCCCACCTAGTCAACGATGGATAGGGGTTCTGAGAGGAAGGTCCCCCACATTGGAACTGAGACACGGTCCAAACTCCTACG | This study |
| HF183/BFDrev IAC | TCATGAGTTCACATGTCCGGGATAATTATTAAAGAATTTCGGTTGTCGATGGGGATGCGTTCCATTAGGCAGTTGGCGGGGTAACGGCCCACTCGAGCTACGATGGATAGGGGTTCCTGCCGTCTCGTGCTCCTCACCACATTGGAACTGAGACACGGTCCAAACTCCTACG | This study |
| HF183/BacR287 IAC | ATCATGAGTTCACATGTCCGCATGATTAAAGGTATTTTCCGGTAGACGATGTGTAGCAACGGCGTGTTATAGTAGGCGGGGTAACGGCCCACCTAGTCAACGATGGATAGGGGTTCTGAGAGGAAG | This study |
a
TAMRA, 6-carboxytetramethylrhodamine.