TABLE 2.

Calibration model performance parameters^a

Assay	Slope	y intercept	R2	E	ROQ	CV (%)		LOD95 (no. of copies)
						10 copies	100 copies
HF183/BFDrev	−3.279	36.07	0.995	1.018	1 to 5	3.56	2.59	100
HF183/BacR287	−3.338	36.74	0.996	0.993	1 to 5	2.45	1.19	10

^aR², coefficient of determination; E, amplification efficiency, calculated as [10^(−1/slope)] − 1; ROQ, range of quantification, based on the log₁₀ number of copies per reaction mixture; CV, coefficient of variation (results for 10 copies and 100 copies of the plasmid standard calculated from 34 to 36 replicate test reactions are shown); LOD₉₅, log₁₀ number of copies of the target per reaction mixture that is detected in at least 95% of replicates (n = 36).