TABLE 3.
Comparison of results obtained with serial dilutions of two human fecal pollution sourcesa
| DNA target type | Assay | Slope | y intercept | R2 | E | LOD95 (pg) |
|---|---|---|---|---|---|---|
| Human fecal DNA | HF183/BFDrev | −3.415 | 24.5 | 0.993 | 0.992 | 1 |
| HF183/BacR287 | −3.432 | 24.6 | 0.992 | 0.956 | 1 | |
| Sewage DNA | HF183/BFDrev | −3.473 | 26.2 | 0.941 | 0.941 | 50 |
| HF183/BacR287 | −3.455 | 26.2 | 0.997 | 0.947 | 5 |
a
R2, coefficient of determination; E, amplification efficiency, calculated as [10(−1/slope)] − 1; LOD95, mass of DNA per reaction mixture that is detected in 95% of replicates (n = 8 for both human fecal DNA and sewage DNA).