FIG 1.

Time course analysis of gene abundance for 16S rRNA, dioxane monooxygenase (DXMO), propane monooxygenase (PrMO), alcohol dehydrogenase (ADH), and aldehyde dehydrogenase (ALDH) gene targets during biotransformation of 1,4-dioxane by pure cultures of Pseudonocardia dioxanivorans CB1190 following enrichment on AMS medium supplemented with 20 mM glucose. Enrichment on glucose increased the lag time before 1,4-dioxane biodegradation was observed. The abundance of all gene targets increased due to increase in CB1190 populations. This analysis also revealed a lag phase before and after 1,4-dioxane biodegradation, which suggests growth on a 1,4-dioxane breakdown product. Error bars represent the standard deviations of three replicates.