TABLE 1.

Sequences of oligonucleotide primers used in this study^a

Target gene/target enzyme	Primer	Sequence (5′→3′)
dxmB/dioxane monooxygenase (DXMO)	DXMO-For	CCAAACGGGCGTCAGTCAT
	DXMO-Rev	AGAACGTGCGCTCCCAAAG
poxD/phenol-2-monooxygenase (PHE)	PHE-For	TGGTCCGGCGAGCCCTTGTA
	PHE-Rev	CACACCTGCTCCGACGGCTG
prm1A/propane monooxygenase (PrMO)	PrMO-For	GAAGAGTCGTGGAAGCAGATC
	PrMO-Rev	GTACTTGTACTCGAACCACTCG
sad/aldehyde dehydrogenase (ALDH)	ALDH-For	GCCGACGCTTTTAGCAGATG
	ALDH-Rev	TCATTAACGGCAGCAAACGC
adh/alcohol dehydrogenase (ADH)	ADH-For	ACCAAGGACCTCACCTCGTA
	ADH-Rev	AACGGATGCGCGTTGTTC
CB1190-like 16S rRNA	CB119016S-For	ACGGTCTCGCAGCCCTCTGT
	CB119016S-Rev	AGCGGGTTATGCCGGGGACT
Universal 16S rRNA	Uni16S-For	ATGGCTGTCGTCAGCT
	Uni16S-Rev	ACGGGCGGTGTGTAC

^aAll primers were designed based on gene targets found in Pseudonocardia dioxanivorans CB1190 (GenBank accession no. NC_015312 and NC_016601), with the exception of the Universal 16S rRNA primers, which were adapted from Harms et al. (43).