FIG 3.

SDS-PAGE analysis of proteins extracted from the supernatant after the disruption of E. coli cells. Proteins in lanes 1 to 4 were extracted from the supernatant after cell disruption; proteins in lanes 5 to 8 were extracted from cellular debris. The extracts obtained from the recombinants were electrophoresed on a 12% polyacrylamide gel under denaturing conditions. The images from SDS-PAGE analyses were captured using the Gel Doc system (Bio-Rad). Lanes 1 and 5, pACYCDuet-1; lanes 2 and 6, pACYC-tCPR; lanes 3 and 7, pACYC-tF3′H; lanes 4 and 8, pACYC-tF3′H-tCPR.