FIG 4.

Schematic diagrams of the hybrid genes used in this study. Green fluorescent protein (GFP) was N-terminally extended by (M)AVTS and fused to a large linker, either inactive PhaC or NanA, which was also fused to genes encoding functions of interest. The hybrid genes were then expressed in E. coli. The resulting fluorescent protein particles were able to display active enzyme as demonstrated by N-acetyl neuraminic acid aldolase (NanA), organophosphohydrolase (OpdA), and thermostable α-amylase (BLA). L, linker.