Table 1.
Predicted protein sequences of arthropod 5-HT2β and 5-HT1α receptors are well conserved
| 2BHum | 2βDro | 2βPan | 2βPro | 1αHel | 1αDro | 1AHum | 1αPan | 1αPro | |
|---|---|---|---|---|---|---|---|---|---|
| 2BHum | X | X | X | X | X | X | X | X | X |
| 2βDro | 31 (47) | X | X | X | X | X | X | X | X |
| 2βPan | 29 (43) | 46 (68) | X | X | X | X | X | X | X |
| 2βPro | 28 (43) | 45 (68) | 72 (97) | X | X | X | X | X | X |
| 1αHel | 21 (33) | 21 (35) | 16 (34) | 16 (34) | X | X | X | X | X |
| 1αDro | 22 (33) | 25 (37) | 17 (33) | 18 (33) | 60 (81) | X | X | X | X |
| 1AHum | 21 (33) | 21 (36) | 15 (33) | 15 (33) | 33 (50) | 18 (49) | X | X | X |
| 1αPan | 22 (34) | 21 (36) | 14 (31) | 15 (32) | 53 (77) | 29 (76) | 36 (50) | X | X |
| 1αPro | 22 (34) | 22 (36) | 14 (31) | 14 (32) | 53 (77) | 30 (77) | 37 (49) | 90 (98) | X |
| 1αPap | 21 (33) | 21 (34) | 16 (33) | 16 (34) | 92 (98) | 35 (82) | 37 (50) | 56 (76) | 53 (77) |
Percentage identities between 5-HT2 and 5-HT1 receptors from Human (Hum), Drosophila (Dro), Panulirus (Pan) and Procambarus (Pro). 5-HT1α receptors from two other arthropods, the tobacco budworm Heliothis virescens (Hel) and the swallowtail butterfly Papilio xuthus (Pap), are also included. Identity was determined for the entire protein and the core region (parentheses) using the ClustalW algorithm in MegAlign (Lasergene). Comparisons amongst 5-HT2 and 5-HT1 receptors are shaded and crustacean ortholog comparisons are in bold. Accession numbers are human (5-HT2BHum, NM_000868; 5-HT1A, AAH69159), Drosophila (5-HT2βDro, NP_731257 plus NP_649805; 5-HT1αDro P28285), Panulirus (5-HT 2βPan, AY550910; 5-HT1αPan, AY528823), Heliothis (5-HT1Hel, CAA64863), Papilio (5-HT1Pap, BAD72868), Procambarus (5-HT2βPro, EU131666; 5-HT1αPro, EU131667).