Reduced skin surface lipids in Cidea−/− mice. (A and C) Thin-layer chromatography (TLC) analysis of skin surface lipids from both 3-month-old and 10-month-old wild-type (+/+) and Cidea−/− (−/−) male (A) and female (C) mice. S, lipid standards. CE, cholesterol esters. WDE, wax diesters. TAG, triglyceride. FFA, free fatty acid. Cho, cholesterol. (B and D) Quantifications of male (B) and female (D) mice. n = 5 for each male genotype. n = 4 for each female genotype. Bars represent means ± SEM. *, P < 0.05; **, P < 0.001; ***, P < 0.0001.