Table 1.

Data and refinement statistics.

	Apo	cGMP bound High-resolution	cGMP-Bound (Iodine SAD)
Data collection
Wavelength (Å)	0.97931	0.97931	1.54178
Space group	P3121	P41212	P41212
Cell dimensions
a,b,c (Å)	78.8,78.8,147.6	47.2, 47.2,101.9	47.9,47.9,104.1
α,β,γ (°)	90,90,120	90,90,90	90,90,90
Resolution (Å)	36.9-2.00	23.8-1.65	15.0-1.80
Rsym or Rmerge	11.4(45.1)*	7.7(47.1)	6.0(21.4)
I/σI	10.2(3.4)	20.1(5.5)	32.2/(2.1)
Completeness (%)	99.4(96.4)	99.6(100)	91.2(35.7)
Redundancy	4.8(4.4)	13.5(13.8)	7.2(5.9)
Refinement
Resolution (Å)	36.9 – 2.00	23.8 – 1.65	15.0 – 1.80
No. reflections	36516	14492	19090
Rwork/Rfree†	18.82/22.69	19.74/24.47	18.9/24.4
No. atoms
Proteins	2934	985	985
Ligand/ion	14	46	45
Water	343	68	56
B-factors
Protein	46.424	28.464	37.069
Ligand/ion	83.734	32.253	49.169
Water	45.140	38.431	37.139
R.m.s. deviations
Bond lengths (Å)	0.006	0.006	0.007
Bond angles (°)	0.980	1.093	1.149

^*Highest resolution shell is shown in parenthesis.

^†5.0% of the observed intensities was excluded from refinement for cross validation purposes.