FIG 4.

NCX1 overexpression exacerbates hind-limb pathology in Sgcd^−/− mice. (A) MW/TL ratios measured in Sgcd^−/− and Sgcd^−/− NCX1 TG mice at 6 months of age. TA, tibialis anterior; Dia., diaphragm. *, P < 0.05 versus Sgcd^−/− mice. (B) Hydroxyproline content of collagen in quadriceps of Sgcd^−/− and Sgcd^−/− NCX1 TG mice measured at 6 weeks and 6 months of age. *, P < 0.05 versus Sgcd^−/− mice. (C) Histological measurements of areas replaced by adipose tissue in quadriceps muscles of Sgcd^−/− and Sgcd^−/− NCX1 TG mice at 6 months of age. *, P < 0.05 versus Sgcd^−/− mice. (D) Quantification of TUNEL-positive muscle fibers between WT, NCX1 TG, Sgcd^−/−, and Sgcd^−/− NCX1 TG mice at 6 weeks of age in histological sections from quadriceps. *, P < 0.05 versus WT mice; #, P < 0.05 versus Sgcd^−/− mice. (E) Representative H&E and Masson's trichrome (Tri) images of quadriceps muscles from Sgcd^−/− and Sgcd^−/− NCX1 TG mice at 6 months of age. (F) Survival plot of Sgcd^−/− and Sgcd^−/− NCX1 TG mice. The error bars indicate SEM.