FIG 9.

NCX1 overexpression rescues histopathology of the diaphragms of mdx mice. (A) MW/TL ratios measured at 6 months of age in WT, NCX1 TG, mdx, and mdx NCX1TG mice for the indicted muscles. *, P < 0.05 versus WT mice; #, P < 0.05 versus mdx mice. (B) Representative H&E-stained histological images (×40 magnification) of diaphragms from mdx and mdx NCX1 TG mice at 2 months of age. (C) Percent fibrosis measured from Masson's trichrome-stained histological sections of diaphragms from mdx and mdx NCX1 TG mice. *, P < 0.05 versus mdx mice. (D) Percentages of fibers with centrally localized nuclei from diaphragm histological sections at 6 months of age in mdx and mdx NCX1 TG mice. *, P < 0.05 versus mdx mice. (E) Western blots for the indicated proteins from quadriceps versus diaphragm in WT mice at 6 weeks of age. DHPR, L-type Ca²⁺ channel. The error bars indicate SEM. Numbers in the bars represent the number of mice analyzed.