FIG 7.

PGC-1 coactivators functionally complex with nuclear receptors TR2 and/or TR4. (A) Diagram depicting the interaction of PGC-1 and TR4 binding on a DR element. HEK293T cells were cotransfected with a luciferase reporter gene directed in cis by 5 copies of a synthetic DR1 sequence (B), the ε-globin gene promoter (containing 2 DR1 elements) (C), or the thymidine kinase (tk) gene promoter (bearing no DR1 sequences) (D) and all possible combinations of PGC-1α, PGC-1β, and TR2 or TR4 expression plasmids. The values for firefly luciferase activity were normalized to cotransfected Renilla luciferase activity. The standard errors were calculated from six independent determinations.