FIG 3.

Renal tubular cell-specific conditional deletion of Gata2 gene (Gata2-CKO). (A) Experimental time course of doxycycline (dox)-induced tubular cell-specific Gata2 deletion. (B) Cre activity indicated by tdTomato are observed exclusively in the tubular cells (T) but not in the glomeruli (G) of the kidney in the Pax8-rtTA::tetO-Cre::Rosa26TdTomato (R26T) reporter transgenic mice. Scale bars, 100 μm. (C) Schematic diagram of Gata2^GFP, Gata2^flox and recombined Gata2⁻ alleles. Primer pairs used to detect the Gata2^flox allele are depicted. (D) Genomic quantitative PCR analysis of the unrecombined Gata2^flox allele in the collecting duct (CD), spleen (SP), and bone marrow (BM) cells. Data are shown as means ± SD derived from three independent experiments. (E) Gata2 mRNA expression level in the CD, SP, and BM cells of Gata2^flox/GFP control and Gata2-CKO mice. Statistical significance of differences between Gata2^flox/GFP (n = 3) and Gata2-CKO (n = 3) mice is depicted (***, P < 0.001; *, P < 0.05; N.S., not significant).