TABLE 1.
Sequence of primers used in quantitative genomic PCR, qRT-PCR, and genotyping
| Gene or genomic element | Sense primer | Antisense primer | Assay |
|---|---|---|---|
| Gata2 | ACCTGTGCAATGCCTGTGGG | TTGCACAACAGGTGCCCGCT | qRT-PCR |
| Gata3 | GGTGGACGTACTTTTTAACAT CGA | CCCTGACGGAGTTTCCGTAG | qRT-PCR |
| Ae1 (Slc4a1) | TATGGGGTCGCCCACATCTAT | AGGCCGAATCTGATCCTCGTA | qRT-PCR |
| Ae4 (Slc4a9) | CCAATTTCCTGGGCATCA | GGGCATCGGGATGAACTT | qRT-PCR |
| Aqp2 | CAGCTCGAAGGAAGGAGACA | GCATTGGCACCCTGGTTCA | qRT-PCR |
| Aqp3 | CTGGGGACCCTCATCCTT | TGGTGAGGAAGCCACCAT | qRT-PCR |
| Aqp4 | TGGAGGATTGGGAGTCACC | TGAACACCAACTGGAAAGTGA | qRT-PCR |
| Creb1 | CCAAACTAGCAGTGGGCAGT | CCCCATCCGTACCATTGTT | qRT-PCR |
| NCC (Slc12a3) | CCTCCATCACCAACTCACCT | CCGCCCACTTGCTGTAGTA | qRT-PCR |
| Nkcc2 (Slc12a1) | ATGCCTCGTATGCCAAATCT | CCCACATGTTGTAAATCCCATA | qRT-PCR |
| Sglt1 (Slc5a1) | CTGGCAGGCCGAAGTATG | TTCCAATGTTACTGGCAAAGAG | qRT-PCR |
| Sglt2 (Slc5a2) | GCTGGATTTGAGTGGAATGC | CGGTCAGATACACTGGCACA | qRT-PCR |
| Umod | CTCAGTGTCCAAGGCTGCTT | GGAAACAACAGCAGCCAGAT | qRT-PCR |
| Avpr2 | GGTCTCGGTCATCCAGTAGC | CTGGTGTCTACCACGTCTGC | qRT-PCR |
| Gapdh | GTCGTGGAGTCTACTGGTGTCTT | GAGATGATGACCCTTTTGGC | qRT-PCR |
| Gata2flox | AGGGACCGGGTACCATAACT | CCTCTAACCCTTCCCTGTCC | Genomic qPCR |
| Aqp2 promoter | CGAGGAAAACAGAGACGTCAA | AAGGCCTATCACCCCATCTT | Genomic qPCR |
| G1-14.4 kb | CAGGGCACAGCGAGTTTAGAG | CCTGTCCTTGGAGCTTGTGAA | Genomic qPCR |
| G1-3.8 kb | CCCTTATCTATGCCTTCCCA | GTGCAAGGCCCAGAAGTC | Genomic qPCR |
| G2-2.8 kb | GCCCTGTACAACCCCATTCTC | TTGTTCCCGGCGAAGATAAT | Genomic qPCR |
| GATA2 flox | TCCGTGGGACCTGTTTCCTTAC | GCCTGCGTCCTCCAACACCTCTAA | Genotyping |
| GATA2 GFP | CTGAAGTTCATCTGCACCACC | GAAGTTGTACTCCAGCTTGTGC | Genotyping |
| Pax8-rtTA | CCATGTCTAGACTGGACAAGA | CTCCAGGCCACATATGATTAG | Genotyping |
| TRE-Cre | ACGTTCACCGGCATCAACGT | CTGCATTACCGGTCGATGCA | Genotyping |