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. 2014 Jun;34(11):2062–2074. doi: 10.1128/MCB.00197-14

FIG 2.

FIG 2

USP28 depletion has minor effects on the dynamics of DDR factors at IRIF. (A) FRAP analysis of GFP-MDC1 in U2OS cells stably expressing the fusion protein transfected with either siLuc (n = 13) or siUSP28 (n = 21). (B) FRAP analysis of GFP-53BP1 in U2OS cells stably expressing GFP-53BP1 transfected with either siLuc (n = 16) or siUSP28 (n = 15). Error bars represent standard deviations. (C to H) Quantification of IRIF numbers via use of antibodies against 53BP1, γH2AX, or conjugated ubiquitin adducts (FK2) following treatment of RPE-1 cells (C, D, and E) or MEFs (F, G, and H) of the indicated genotypes with 2 Gy of IR, viewed with an Opera microscope. MEF data represent the averages of 2 experiments, each using 2 littermate pairs of WT and Usp28-deficient MEFs.