FIG 3.
Chromatin remodelers functionally interact with Smc5/6 and Top2. (A) swr1Δ suppresses the HU and MMS sensitivities of smc6-74. Spots are 10-fold serial dilutions grown at 30°C for 4 days. (B) swr1Δ is synthetically lethal with top2-191. Plates were incubated at 25°C (5 days) or 30°C (4 days). (C) Western blot analysis of total and acetylated Pht1 (Pht1Ac). Tubulin was used as a loading control. The dotted line indicates the boundary of omitted lanes between the wild-type and swr1Δ samples. (D) arp8Δ suppresses the HU and MMS sensitivities of smc6-74. Spots are 10-fold serial dilutions, and plates were incubated at 30°C for 4 days. (E) arp8Δ is synthetically lethal with top2-191 at 30°C. Plates were grown at 25°C for 5 days or for 4 days at 30°C and 36°C. (F) DAPI-stained DIC images of wild-type and arp8Δ cells grown at 25°C and 36°C. Bar, 10 μm.