FIG 3.

Arsenite-induced AVGs recruit components similar to those recruited by ΔE3L-VACV-induced AVGs. HeLa cells were either mock infected (A to C) or infected with WT-VACV (D to I) or ΔE3L-VACV (J to L) at an MOI of 10. PBS (A to F and J to L) or 500 μM arsenite (G to I) was added at 6 hpi, and cells were fixed an hour later. Triplicate coverslips were stained with antibodies against either G3BP1 and eIF3H (A, D, G, and J), TIA1 and eIF4E (B, E, H, and K), or CAPRIN1 and PABP (C, F, I, and L) and stained with DAPI. Maximum-intensity projections from multiple z-slices. Boxed areas mark zoomed regions. Bar = 5 μm.