FIG 4.

Arsenite-induced stress granules but not antiviral granules disassemble during elongation inhibition. HeLa cells were infected with WT-VACV at an MOI of 10 or mock infected and left untreated (A and G), treated with 0.5 mM arsenite for 30 min (B and H), treated with 10 μg/ml cycloheximide for 1 h (C and I), treated with arsenite for 90 min with the addition of cycloheximide after 30 min (D and J), treated with 20 μg/ml puromycin for 1 h (E and K), or treated with arsenite for 90 min with the addition of puromycin after 30 min (F and L). Cells were fixed at 7 hpi and stained with antibodies to CAPRIN1 (red) and with DAPI (blue). Fields of view were collected at a ×63 magnification. Arrows mark viral factories with AVGs, white arrowheads mark factories without AVGs, and open arrowheads mark SGs. Bar = 5 μm. Quantification of infected cells showing factory-associated CAPRIN1 granules (A to F) or of all cells with stress granules (G to L) is shown in each panel (n = 100 each).