FIG 4.

Infection of Kasumi-3 cells with virus lacking the miRNA-binding site in the UL122 (IE2) 3′ UTR favors lytic replication. Kasumi-3 cells were infected with wild-type TB40/Egfp (WT), TB40/EgfpIE2cisΔ (IE2cisΔ), or TB40/EgfpIE2cisΔrep (IE2cisΔRep) virus. (A) Viral gene expression was assessed at 7 dpi by RT-qPCR with primers directed at UL123. All samples were analyzed in triplicate, and levels were normalized to the level of GAPDH gene production. (B) Extracellular virion production was assessed over a 5-day time course. Viral genomes derived from the supernatants of the infected cells were analyzed by qPCR using primers that detect UL123. All samples were analyzed in triplicate. AU, arbitrary units.