FIG 3.

Production of HHV-6 proteins in primary murine glial brain cultures overlaid with HHV-6A-infected HSB2 lymphocytes. Primary murine brain glial cells generated from CD46-transgenic (CD46-cyt1) mice were cocultured with HHV-6A-infected HSB2 cells. Seven days after the establishment of coculture, supernatants as well as nonadherent cells, were removed, and adherent cells were fixed and analyzed for the presence of viral antigens by confocal microscopy. Cells were stained with antibodies against HHV-6 protein (green) p41 (A to C) or gp116 (D) or with glial fibrillary acidic protein (GFAP) antibody (red), and cell nuclei were stained with DAPI (blue). With p41 antibody, both cytoplasmic (A and B) and nuclear (C) staining was observed. Scale bar, 10 μm.