TABLE 1.
HHV-6 specific primers used in qPCR analyses
| Target genea | Sequence |
Amplicon length (bp) | |
|---|---|---|---|
| Forward | Reverse | ||
| Genomic DNA templates | |||
| U41 | 5′-GTCATAGACCGGAGCATCGT-3′ | 5′-TGAGGTGATGAGGGATAGGG-3′ | 196 |
| Murine β-actin | 5′-GGTACTAACAATGGCTCGTGTGAC-3′ | 5′-TCAGGGCAGGTGAAACTGTATGG-3′ | 123 |
| Human β-globin | 5′-CCCTTGGACCCAGAGGTTCT-3′ | 5′-CGAGCACTTTCTTGCCATGA-3′ | 101 |
| cDNA templates | |||
| U79 (E) | 5′-AACGACGAAGACAAGCAACCG-3′ | 5′-TGTTATGCCATCCTCGTGACTTTG-3′ | 123 |
| U86 (IE2, LAT) | 5′-ACCCACAGACAATGCACATCC-3′ | 5′-TGGGCTGTAGGAGTTGATTCG-3′ | 106 |
| U90 (IE1, LAT) | 5′-TGGAAACACAGACCCATCAGAC-3′ | 5′-TCTGGAGAAGGAGTGCATGGAT-3′ | 141 |
| U94 (IE, LAT) | 5′-CGCCCCTGATTTCCGTTGTG-3′ | 5′-CCTGCAAAGTGGTACGCTCAAG-3′ | 121 |
| U100 (L) | 5′-AACTGAGGAACTGATGGAAACC-3′ | 5′-GCTTACGATGGCAGGATCTATG-3′ | 109 |
| Murine CCL5 | 5′-GCACCTGCCTCACCATATGG-3′ | 5′-AGCACTTGCTGCTGGTGTAG-3′ | 112 |
| Murine CCL2 | 5′-ACCAGCACCAGCCAACTC-3′ | 5′-CAGAAGCATGACAGGGACC-3′ | 75 |
| Murine CXCL10 | 5′-GCAACTGCATCCATATCG-3′ | 5′-GACATCTCTGCTCATCATTC-3′ | 135 |
| Murine GAPDH | 5′-GCATGGCCTTCCGTGTCC-3′ | 5′-TGTCATCATACTTGGCAGGTTTCT-3′ | 84 |
| Human GAPDH | 5′-CACCCACTCCTCCACCTTTGAC-3′ | 5′-GTCCACCACCCTGTTGCTGTAG-3′ | 112 |
a
E, early; IE, immediate early; L, late; LAT, latency-associated transcript.