FIG 1.

SIV MA tube formation is mediated by CXCR1 and CXCR2. (A) Capillary-like structure formation of HUVECs is shown in response to the indicated treatments. HUVECs were seeded on BME and stimulated at 37°C with PBS (not treated cells [NT]) or 10 ng of GST, p17, or SIV MA/ml. (B) SIV MA-induced capillary-like structure formation is mediated specifically by both CXCR1 and CXCR2. HUVECs were stimulated at 37°C with PBS (NT) or SIV MA (10 ng/ml) after preincubation of viral protein with 2.5 μg/ml of a neutralizing MAb to CXCR1, a neutralizing MAb to CXCR2, a combination of MAb to CXCR1 and CXCR2, or a control isotype-matched MAb (Ctrl MAb). Pictures were taken after 8 h of culture on BME (original magnification, ×10) and are representative of three independent experiments with similar results. Values reported for HUVEC tube formation are means ± the SD of three independent experiments. Statistical analysis was performed by one-way ANOVA, and a Bonferroni's post test was used to compare data (***, P < 0.001).