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. 2014 May;88(10):5706–5717. doi: 10.1128/JVI.03142-13

FIG 3.

FIG 3

p17 and SIV MA induce CXCR2-mediated B cell migration. (A and B) Transwell migration assay of B cells in response to PBS (NT), IL-8, p17, or SIV MA at the indicated concentrations. Bars represent the means ± the SD of three independent experiments performed in duplicate. Statistical analysis was performed by one-way ANOVA, and a Bonferroni's post test was used to compare data (***, P < 0.001). (C and D) Transwell migration assay of B cells in response to the indicated treatments. B cells were stimulated for 90 min at 37°C with PBS (NT) or pretreated for 1 h at 37°C with 50 μg of MAb to CXCR1, MAb to CXCR2, or Ctrl MAb/ml and then stimulated for 90 min at 37°C with p17 or SIV MA (100 ng/ml). Bars represent the means ± the SD of three independent experiments performed in duplicate. Statistical analysis was performed by one-way ANOVA, and a Bonferroni's post-test was used to compare data (*, P < 0.05; **, P < 0.01). (E) Transwell migration assay of B cells nucleoporated with CXCR2 siRNAs or with scr siRNAs in response to the indicated treatments. Bars represent the means ± the SD of three independent experiments performed in duplicate. Statistical analysis was performed by using a paired two-tailed Student t test (**, P < 0.01). (F) Transwell migration assay of Jurkat cells transfected with pEGFP-N3 or pEGFP-N3-CXCR2 in response to the indicated treatments. Bars represent the means ± the SD of three independent experiments performed in duplicate. Statistical analysis was performed by one-way ANOVA, and a Bonferroni's post test was used to compare data (**, P < 0.01; ***, P < 0.001).