FIG 5.

Effect of p17, S75X, and SIV MA on pERK1/2 and pAkt in Raji and B cells. (A and B) Cells were treated for 5 min with p17, S75X, and SIV MA at a concentration of 0.1 μg/ml. Untreated cells were used as a control. Western blot analysis of Raji lysates shows that p17 inhibited the activation of Akt and induced the activation of ERK1/2, whereas S75X and SIV MA activated both Akt and ERK1/2, as verified by densitometric analysis and plotting of the pAkt/ERK1/2 and pERK1/2/ERK1/2. (Upper panels) Blots from one representative experiment of three with similar results are shown. (Lower panels) Values reported for pERK1/2 and pAkt are the means ± the SD of three independent experiments. Statistical analysis was performed by one-way ANOVA, and a Bonferroni's post test was used to compare data (*, P < 0.05; **, P < 0.01; ***, P < 0.001).