FIG 1.
DHBTs are potent inhibitors of DENV2 infection. (A) Structure of shared DHBT scaffold of DENV2 inhibitors. R groups R1 and R2 may be located anywhere on their respective rings. (B) Structure of SKI-417616. (C) Dose-response curve for SKI-417616 and DENV-Luc in HEK293 cells. (D) HEK293 cells were infected with DENV2 at an MOI of 0.1 FFU/cell in the presence of 1 μM or 10 μM SKI-417616 or DMSO (control). Supernatants were collected at the indicated times p.i., and titers were determined on Vero cells. Significant (P, <0.05 by a two-tailed t test) inhibition by 1 μM and 10 μM SKI-417616 (compared to virus levels with the DMSO control) was detected at days 2 and 3. (E) Differentiated THP1 cells were infected with DENV2 at an MOI of 10 FFU/cell in the presence of 10 μM SKI-417616. Supernatants were collected at the indicated times p.i., and titers were determined on Vero cells. *, P < 0.05; **, P < 0.01.