FIG 5.
DHBTs inhibit DENV2 infection through antagonism of ERK signaling via DRD4. (A and B) Serum-starved HEK293 cells were treated with the indicated compound at 5 μM. Total protein (A) or total RNA (B) was isolated at 10 min (A) or 2 h (B) posttreatment. (A) SDS-PAGE and Western blot analysis were performed to detect phospho-ERK (top) and total ERK (bottom). (B) c-fos and β-actin levels were determined by RT-qPCR. The fold change in the c-fos level was calculated by ΔΔCT analysis. (C) HEK293 cells were infected with DENV2 at an MOI of 0.1 FFU/cell with increasing concentrations of U0126 (1 to 10 μM). Supernatants were collected at 48 h p.i., and titers were determined on Vero cells.