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Quantification of acquisition of TYLCV in Rick+ and Rick− whitefly strains using a qPCR approach. The quantification was performed relative to actin, a housekeeping gene that did not change over time under these conditions. Whiteflies were collected from a colony continuously reared on TYLCV-infected tomato plants (A) or reared on TYLCV-infected tomato plants for 48 h (B).
