FIG 2.

Viral protein expression during MVA, ANK, WR, NYBH, NYVAC, or CopV infection. Primary human macrophages were mock infected or infected with the indicated poxviruses (5 PFU/cell), and at the indicated times p.i., equal amounts of proteins from cell extracts were fractionated by SDS-PAGE, transferred to nitrocellulose membranes, and treated with antibodies to specific virus early (p25) and late proteins (p64 and p14). Molecular weight (MW; in thousands) is indicated based on protein standards. In all blots, detection of actin was used as a loading control.