FIG 5.

Quantification of virus titer in Nicotiana benthamiana and Datura stramonium suggests the former as a better acquisition host in transmission experiments with thrips. Plants were mechanically inoculated with isolates p105 and p105RBMar (A), p105 and p105-803RB (B), and p202/3WT and p202/3RB (C). Systemically infected leaves displaying severe symptoms were sampled 7 days postinoculation and used for acquisition (72 h). Double antibody sandwich-enzyme linked immunosorbent assay was performed using the antinucleocapsid antibody A421VI. A five-point standard curve prepared from sap of a pool of p105-infected leaves from the same plants (undiluted and 80%, 60%, 40%, and 20% dilutions with sap from healthy plants) was used for relative virus titer determination based on absorbance values. Statistical analysis was performed by ANOVA using SigmaStat analysis software v. 3.5. An asterisk indicates that differences are statistically significant (P < 0.05).